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Health, Exercise, and Sports Sciences ETDs
Publication Date
Spring 5-15-2024
Abstract
Stimulated contractions are shown to increase toll-like receptor 4 (TLR4) release from C2C12 myotubes in vitro. The purpose of this study was to determine if the release of TLR4 from myotubes is controlled by proteolytic membrane cleavage. A broad-spectrum metalloprotease inhibitor was used to test this hypothesis in a C2C12 cell culture model, and TLR4 concentrations in the cell culture supernatant were measured via enzyme-linked immunosorbent assay (ELISA) following stimulated myotube contractions.
ELISA analysis revealed TLR4 concentrations below the sensitivity threshold of the ELISA kit for all experimental conditions. In confirming the effectiveness of the electrical pulse stimulation (EPS) treatment, ANOVA analysis showed myotube diameter was not significantly different between conditions (p > 0.05), and supernatant IL-6 concentrations were not increased with EPS treatment. These results reflect an abnormal response of the C2C12 myotubes to stimulated contractions here, and additional troubleshooting is needed to test the proposed hypothesis.
Keywords
Type II Diabetes Mellitus, insulin resistance, toll-like receptor 4, metalloprotease, skeletal muscle, C2C12 myotube
Document Type
Thesis
Language
English
Degree Name
Physical Education
Level of Degree
Masters
Department Name
Health, Exercise, and Sports Sciences
First Committee Member (Chair)
Michael Deyhle
Second Committee Member
Christine Mermier
Third Committee Member
Judy Cannon
Recommended Citation
Gridley, Rebekah C.. "The enzymatic regulation of contraction-induced toll-like receptor 4 shedding from C2C12 myotubes." (2024). https://digitalrepository.unm.edu/educ_hess_etds/186
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Health and Physical Education Commons, Nutritional and Metabolic Diseases Commons, Other Medicine and Health Sciences Commons, Translational Medical Research Commons