Health, Exercise, and Sports Sciences ETDs

Publication Date

Spring 5-15-2024

Abstract

Stimulated contractions are shown to increase toll-like receptor 4 (TLR4) release from C2C12 myotubes in vitro. The purpose of this study was to determine if the release of TLR4 from myotubes is controlled by proteolytic membrane cleavage. A broad-spectrum metalloprotease inhibitor was used to test this hypothesis in a C2C12 cell culture model, and TLR4 concentrations in the cell culture supernatant were measured via enzyme-linked immunosorbent assay (ELISA) following stimulated myotube contractions.

ELISA analysis revealed TLR4 concentrations below the sensitivity threshold of the ELISA kit for all experimental conditions. In confirming the effectiveness of the electrical pulse stimulation (EPS) treatment, ANOVA analysis showed myotube diameter was not significantly different between conditions (p > 0.05), and supernatant IL-6 concentrations were not increased with EPS treatment. These results reflect an abnormal response of the C2C12 myotubes to stimulated contractions here, and additional troubleshooting is needed to test the proposed hypothesis.

Keywords

Type II Diabetes Mellitus, insulin resistance, toll-like receptor 4, metalloprotease, skeletal muscle, C2C12 myotube

Document Type

Thesis

Language

English

Degree Name

Physical Education

Level of Degree

Masters

Department Name

Health, Exercise, and Sports Sciences

First Committee Member (Chair)

Michael Deyhle

Second Committee Member

Christine Mermier

Third Committee Member

Judy Cannon

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