Dental Hygiene ETDs

Publication Date

Summer 8-1-2023

Abstract

Oral plaque biofilm has been identified as the primary contributor to dental conditions such as dental caries and periodontal disease that promotes a diverse and pervasive collection of microbial species to inhabit the oral cavity. Disrupting the plaque matrix early could help combat this global disease burden. This study evaluated the effect of IONPs on primary gingival keratinocytes.

Purpose: This study aimed to examine existing research regarding nanoparticles and determine if IONPs affect the PGK cell membranes with preliminary research to find the maximum lysis possible with PGK cells.

Methods: IONP core/shell syntheses were performed. Considering keratinocyte characteristics, examining data regarding metabolic activity and cell death was important preliminary data. An LDH-based colorimetry assay was selected; data was measured with a colorimetry absorbance plate reader at 490nm. Values were plotted.

Conclusions: The calibration curve shows when controlled with varying materials, absorbance values represent a suitable linear and concentration-dependent baseline for future nanoparticle lysis comparison.

Degree Name

Dental Hygiene

Level of Degree

Masters

Department Name

Dental Medicine

First Committee Member (Chair)

Christine Nathe RDH, MS

Second Committee Member

Marek Osinski Ph.D, MSc

Third Committee Member

Robin Gatlin RDH, MS

Fourth Committee Member

Justine Ponce RDH, MS

Language

English

Keywords

Iron Oxide Nanoparticles, Oral Health Prevention, Nanotechnology, Oral Biofilms, F. nucleatum, SPIONs

Document Type

Thesis

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