Biomedical Sciences ETDs
Publication Date
Fall 12-14-2024
Abstract
This study aimed to investigate the efficiency of saliva analysis in detecting SARS-CoV-2 through a clinical investigation involving 20-25 participants. The study evaluated a rapid protease activity test in saliva compared to an FDA-authorized RT-PCR test. Eligible participants, aged 18 years or older, underwent routine RT-PCR testing. This protease test represents an alternative to nucleic acid and antigen tests, potentially suitable for POCT. Assessment criteria included PPA and NPA compared to RT-PCR results, with exploration of false positives and negatives. Subjects with discordant results were retested after 72 hours to assess viral detectability. Additionally, we delved into the genome organization of SARS-CoV-2 and the proteins roles, MPro and PLPro, in viral replication. Biochemical methods optimized a protease test utilizing specific peptide substrates, demonstrating high sensitivity in detecting viral activity in saliva. This research highlights saliva testing's promise in SARS-CoV-2 detection, contributing insights into disease management strategies and global health implications.
Keywords
Protease, POCT, MPro, PLpro, SARS-CoV-2, Saliva
Sponsors
Mesa Photonics Lab
Document Type
Thesis
Language
English
Degree Name
Biomedical Sciences
Level of Degree
Masters
Department Name
Biomedical Sciences Graduate Program
First Committee Member (Chair)
Hua-Ying Fan, PhD
Second Committee Member
Justin T Baca, PhD, MD
Third Committee Member
Frauke Rininsland, PhD
Recommended Citation
Jimenez, Anahi G.. "THE DEVELOPMENT OF A NOVEL PROTEASE ASSAY POINT-OF-CARE TEST FOR SARS-COV-2 IN SALIVA." (2024). https://digitalrepository.unm.edu/biom_etds/267
