Supporting data for Sequential Super-Resolution Imaging using DNA Strand Displacement

Authors

Sandeep PallikkuthFollow
Cheyenne Martin
Farzin Farzam
Jeremy S. Edwards
Matthew R. Lakin
Diane S. Lidke
Keith A. Lidke

Document Type

Dataset

Publication Date

2018

Abstract

Sequential labeling and imaging in super-resolution fluorescence microscopy allows the imaging of multiple structures in the same cell using a single fluorophore species. In this work, we describe a method based on DNA strand displacement that can be used to quickly and easily perform the labeling and removal of the fluorophores during each sequence. Labeling for a particular structure is achieved by hybridization of site-specific antibody-bound ssDNA with a complimentary dye-labeled strand. After imaging, the dye is removed using toehold-mediated strand displacement, in which an invader strand competes off the dye-labeled strand than can be subsequently washed away. We demonstrate the concept using sequential dSTORM super-resolution for multiplex imaging of subcellular structures.

Comments

Fig2: Data validating rapid kinetics of strand displacement and low residual cross-talk fluorescence after invader treatment.

Fig3: Data containing image stacks from six total rounds of sequential imaging of sub-cellular structures in HeLa cell.

Recommended Citation

Pallikkuth, S., Martin, C., Farzam, F., Edwards, J. S., Lakin, M. R., Lidke, D. S., & Lidke, K. A. (2018). Supporting data for Sequential Super-Resolution Imaging using DNA Strand Displacement [Data set]. University of New Mexico. https://doi.org/10.25827/CS2A-DH13

DOI

doi:10.25827/cs2a-dh13