Document Type
Dataset
Publication Date
2018
Abstract
Sequential labeling and imaging in super-resolution fluorescence microscopy allows the imaging of multiple structures in the same cell using a single fluorophore species. In this work, we describe a method based on DNA strand displacement that can be used to quickly and easily perform the labeling and removal of the fluorophores during each sequence. Labeling for a particular structure is achieved by hybridization of site-specific antibody-bound ssDNA with a complimentary dye-labeled strand. After imaging, the dye is removed using toehold-mediated strand displacement, in which an invader strand competes off the dye-labeled strand than can be subsequently washed away. We demonstrate the concept using sequential dSTORM super-resolution for multiplex imaging of subcellular structures.
Recommended Citation
Pallikkuth, S., Martin, C., Farzam, F., Edwards, J. S., Lakin, M. R., Lidke, D. S., & Lidke, K. A. (2018). Supporting data for Sequential Super-Resolution Imaging using DNA Strand Displacement [Data set]. University of New Mexico. https://doi.org/10.25827/CS2A-DH13
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Fig2B-Tubulin.h5 (7820612 kB)
Fig2C-Clathrin.h5 (6779574 kB)
Fig2C-Tubulin.h5 (6833279 kB)
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Fig3A-Tubulin-Round3.h5 (3432711 kB)
DOI
doi:10.25827/cs2a-dh13
Comments
Fig2: Data validating rapid kinetics of strand displacement and low residual cross-talk fluorescence after invader treatment.
Fig3: Data containing image stacks from six total rounds of sequential imaging of sub-cellular structures in HeLa cell.