Authors
Samuel M Goodfellow, Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Robert A. Nofchissey, Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Kurt C. Schwalm, Department of Pediatrics, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Joseph A. Cook, Museumof Southwestern Biology, Biology Department, University of New Mexico, Albuquerque, New Mexico, USA
Jonathan L. Dunnum, Museumof Southwestern Biology, Biology Department, University of New Mexico, Albuquerque, New Mexico, USA
Yan Guo, Comprehensive Cancer Center, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Chunyan Ye, Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Gregory J. Mertz, Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Kartik Chandran, Albert Einstein College of Medicine, Department of Microbiology and Immunology, Bronx, New York, USA
Michelle Harkins, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Daryl B. Domman, Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Darrell L. Dinwiddie, Department of Pediatrics, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Steven B. Bradfute, Center for Global Health, Department of Internal Medicine, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA
Publication Date
11-9-2021
Abstract
Sin Nombre orthohantavirus (SNV), a negative-sense, single-stranded RNA virus that is carried and transmitted by the North American deer mouse Peromyscus maniculatus, can cause infection in humans through inhalation of aerosolized excreta from infected rodents. This infection can lead to hantavirus cardiopulmonary syndrome (HCPS), which has an ∼36% case-fatality rate. We used reverse transcriptase quantitative PCR (RT-qPCR) to confirm SNV infection in a patient and identified SNV in lung tissues in wild-caught rodents from potential sites of exposure. Using viral whole-genome sequencing (WGS), we identified the likely site of transmission and discovered SNV in multiple rodent species not previously known to carry the virus. Here, we report, for the first time, the use of SNV WGS to pinpoint a likely site of human infection and identify SNV simultaneously in multiple rodent species in an area of known host-to-human transmission. These results will impact epidemiology and infection control for hantaviruses by tracing zoonotic transmission and investigating possible novel host reservoirs.
Publisher
American Society For Microbiology
Publication Title
Journal of virology
Recommended Citation
Goodfellow SM, Nofchissey RA, Schwalm KC, Cook JA, Dunnum JL, Guo Y, Ye C, Mertz GJ, Chandran K, Harkins M, Domman DB, Dinwiddie DL, Bradfute SB. Tracing Transmission of Sin Nombre Virus and Discovery of Infection in Multiple Rodent Species. J Virol. 2021 Nov 9;95(23):e0153421. doi: 10.1128/JVI.01534-21. Epub 2021 Sep 22. PMID: 34549977; PMCID: PMC8577387.
