Optical Science and Engineering ETDs

Publication Date

Fall 8-4-2025

Abstract

Advancing biomedical imaging requires platforms that combine high-resolution, speed, and stability across biological scales. This dissertation presents three innovations designed to address limitations of conventional light-sheet and oblique plane imaging. First, we introduce Reflected Inline Detection in Epi-Oblique Plane Microscopy (RIDE-OPM), a compact and drift-resistant system that eliminates a tertiary detection path while maintaining alignment flexibility, enabling robust long-term live imaging. Second, we present a high-speed Axially Swept Light-Sheet Microscopy (ASLM) platform for cleared tissues, using dual foci and synchronized sweeping to achieve isotropic sub-micron resolution at up to 40 frames-per-second, a fourfold improvement over standard ASLM. Integrated with a deep-learning tissue boundary detector, it enables efficient 3D imaging. Finally, we propose a remote focusing architecture that ensures consistent magnification and 4f geometry across objectives, enabling seamless multi-scale imaging. Together, these methods establish a flexible framework for next-generation microscopy with broad applications in live, cleared, and multi-resolution imaging.

Degree Name

Optical Science and Engineering

Level of Degree

Doctoral

Department Name

Optical Science and Engineering

First Committee Member (Chair)

Tonmoy Chakraborty

Second Committee Member

Keith A. Lidke

Third Committee Member

Sheng Liu

Fourth Committee Member

Irene Salinas Remiro

Keywords

Light-sheet Microscopy, Volumetric Imaging, Remote Focusing, Oblique Plane, Isotropic Imaging

Document Type

Dissertation

Language

English

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