High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells

Authors

Hanieh Mazloom-Farsibaf, Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, United States
William K. Kanagy, Department of Pathology, University of New Mexico, Albuquerque, NM, United States
Diane S. Lidke, Department of Pathology, University of New Mexico, Albuquerque, NM, United States Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, United States
Keith A. Lidke, Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, United States Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, United States

Document Type

Article

Publication Date

6-1-2020

Abstract

A high-speed fluorescence microscope operating at a 490 Hz frame rate was used to image two different membrane proteins- the high-affinity IgE receptor FcɛRI, a transmembrane protein, and an outer-leaflet GPI-anchored protein. The IgE receptor was imaged via IgE labeled with Janelia Fluor 646 and the GPI-anchored protein was imaged using a GPI-GFP fusion protein and an ATTO 647 N labeled anti-GFP nanobody. Data was collected for both proteins in untreated cells and cells that had actin stabilized by phalloidin. This dataset can be used for development and testing of single-particle tracking methods on experimental data and to explore the hypothesis that the actin cytoskeleton may affect the movement of membrane proteins.

Recommended Citation

Mazloom-Farsibaf H, Kanagy WK, Lidke DS, Lidke KA. High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells. Data Brief. 2020 Apr 6;30:105424. doi: 10.1016/j.dib.2020.105424. PMID: 32322610; PMCID: PMC7168344.