Chemistry and Chemical Biology ETDs

Weimin xuan

Publication Date

9-5-2013

Abstract

Fluorescent probes have been proved to be very valuable tools in chemical and biological applications, and extensive exploration has been made. Recently, reaction-based fluorescent probes became a new design trend and displayed great potential with remarkable implementation to the conventional design method. However, this field is still underdeveloped, and a lot of problems remain to be solved. As part of our groups research interest, my Ph. D. study centered on the development of new reaction-based fluorescent probes. Firstly, our previous research on Hg2+ was continued. In this work, we developed a novel ratiometric fluorescent probe for Hg2+ with impressive sensitivity, selectivity and imaging property; also, a new fluorescent probe with excellent detection limit was explored. Secondly, the first FRET-based ratiometric fluorescent probe for nerve agent was designed and synthesized, and it exhibited great potential for practical application. Furthermore, a new design strategy for H2S probe development was discovered, and the new probe displayed excellent sensitivity and specificity. Finally, probes for Fe2+ were intensively investigated due to their biological significance and urgent demand, and the real time and off-on fluorescence detection of Fe2+ was achieved for the first time.

Language

English

Keywords

fluorescent probe, fluorescence imaging, ratiometric, FRET, coumarin, rhodamine, naphthalimide

Document Type

Dissertation

Degree Name

Chemistry

Level of Degree

Doctoral

Department Name

Department of Chemistry and Chemical Biology

First Committee Member (Chair)

Qin, Yang

Second Committee Member

Feng, Changjian

Third Committee Member

Melancon, Charles

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