Document Type
Article
Publication Date
1-2018
Abstract
Previous reports indicate roles for acid-sensing ion channels (ASICs) in both peripheral and central chemoreception, but the contributions of ASICs to ventilatory drive in conscious, unrestrained animals remain largely unknown. We tested the hypotheses that ASICs contribute to hypoxic- and hypercapnic-ventilatory responses. Blood samples taken from conscious, unrestrained mice chronically instrumented with femoral artery catheters were used to assess arterial O2, CO2, and pH levels during exposure to inspired gas mixtures designed to cause isocapnic hypoxemia or hypercapnia. Whole-body plethysmography was used to monitor ventilatory parameters in conscious, unrestrained ASIC1, ASIC2, or ASIC3 knockout (-/-) and wild-type (WT) mice at baseline, during isocapnic hypoxemia and during hypercapnia. Hypercapnia increased respiratory frequency, tidal volume, and minute ventilation in all groups of mice, but there were no differences between ASIC1-/-, ASIC2-/-, or ASIC3-/- and WT. Isocapnic hypoxemia also increased respiratory frequency, tidal volume, and minute ventilation in all groups of mice. Minute ventilation in ASIC2-/- mice during isocapnic hypoxemia was significantly lower compared to WT, but there were no differences in the responses to isocapnic hypoxemia between ASIC1-/- or ASIC3-/- compared to WT. Surprisingly, these findings show that loss of individual ASIC subunits does not substantially alter hypercapnic or hypoxic ventilatory responses.
Publisher
PLOS ONE
Publication Title
Role of Acid-Sensing Ion Channels in Hypoxia- and Hypercapnia-Induced Ventilatory Responses
Recommended Citation
Detweiler, Neil D.; Kenneth Vigil; Thomas C. Resta; Benjimen R. Walker; and Nikki L. Jernigan. "Role of Acid-Sensing Ion Channels in Hypoxia- and Hypercapnia-Induced Ventilatory Responses." Role of Acid-Sensing Ion Channels in Hypoxia- and Hypercapnia-Induced Ventilatory Responses (2018). https://digitalrepository.unm.edu/cell_pubs/4
plethysmography A1KO vs WT hypercapnia.xlsx (1909 kB)
plethysmography A1KO vs WT isocapnic hypoxia.xlsx (1952 kB)
plethysmography A2KO vs WT hypercapnia.xlsx (2244 kB)
plethysmography A2KO vs WT isocapnic hypoxia.xlsx (1971 kB)
plethysmography A3KO vs WT hypercapnea.xlsx (1250 kB)
plethysmography A3KO vs WT isocapnic hypoxia.xlsx (1332 kB)
