Biomedical Engineering ETDs

Publication Date

Summer 7-29-2025

Abstract

Significant advances in manufacturing technologies have enabled the production of new DNA microarrays for use in emergent technology. Photolithography, wafer scale manufacturing, and improvements in synthesis chemistry and design enable high-throughput production of extremely high-quality arrays. These new arrays offer extremely high oligonucleotide densities, in the range of tens of thousands of oligonucleotides per square micron. They also offer smaller features sizes, with millions of features per square centimeter. Furthermore, they can be manufactured with the DNA tethered at either end to the surface, leaving either the 5’ or 3’ end free and available for use in enzymatic reactions; and they can be manufactured on new surfaces, such as hydrogels, which are biocompatible with enzymatic reactions. Here, we discuss the use of these new arrays for pathogen detection, viral genotyping, and high-resolution spatial transcriptomics.

Language

English

Keywords

microarray, spatial, transcriptomics, pathogen

Document Type

Dissertation

Degree Name

Biomedical Engineering

Level of Degree

Doctoral

Department Name

Biomedical Engineering

First Committee Member (Chair)

Jeremy S. Edwards

Second Committee Member

David G. Whitten

Third Committee Member

Michelle A. Ozbun

Fourth Committee Member

William P. Bricker

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