Biomedical Engineering ETDs

Publication Date

Summer 7-29-2025

Abstract

Aging is a common risk factor for many chronic diseases, such as cancer, neurodegenerative disease, and heart disease. It has been theorized that a better understanding of aging may yield better preventative treatments for chronic disease. The nematode Caenorhabditis elegans is a widely used model organism for aging research, due to its small size, short lifespan, and wide genetic tool kit. However, manual lifespan scoring, though reliable is time consuming and limits scale. Automation offers a solution to increase the productivity researchers, but existing systems often compromise scalability, accuracy, and throughput, making them unfit for experiments at the genomic scale. To address this, I designed The Worm Automation Machine (WAM), an open source, hands free, image-based automation solution for high throughput C. elegans data collection. WAM combines modular design and machine learning to match the throughput of 8 researchers per hour. The WAM pipeline enables the simultaneous collection of lifespan and healthspan data, supporting larger and more efficient experimental workflows that advance our understanding of the fundamental biology of aging.

Language

English

Keywords

C. elegans, Aging, Lifespan, Healthspan, Automation, Machine learning

Document Type

Thesis

Degree Name

Biomedical Engineering

Level of Degree

Masters

Department Name

Biomedical Engineering

First Committee Member (Chair)

Dr. Mark McCormick

Second Committee Member

Dr. Olga Ponomarova

Third Committee Member

Dr. John King

Available for download on Thursday, July 29, 2027

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