CHARACTERIZATION OF PHASE BEHAVIOR IN IDP SOLUTIONS BY UV-VIS SPECTROPHOTOMETRY AND PHASE VOLUME FRACTION MEASUREMENTS IN CAPILLARIES

Author

Qing SunFollow

Publication Date

Winter 12-17-2021

Abstract

Intrinsically disordered proteins (IDPs) play vital roles in cell signaling and regulation by changing conformation dynamically under various stimulus. Thus, it is crucial to understand their physical properties in vitro especially liquid-liquid phase separation (LLPS). Here, we first used UV-vis spectrophotometry to investigate two of IDPs' physical properties – transition temperature (Tt) and coacervate size. This deeper insight allows us to further understand the dynamics of phase growth. We then constructed a binodal curve in the phase diagram by measurement of volume ratios in phase separated IDP solution in glass capillaries.

Language

English

Keywords

Phase Diagram; ELP; Transition Temperature; Binodal Curve

Document Type

Thesis

Degree Name

Biomedical Engineering

Level of Degree

Masters

Department Name

Biomedical Engineering

First Committee Member (Chair)

Nick Carroll

Second Committee Member

Gabriel Lopez

Third Committee Member

Andrew Shreve

Recommended Citation

Sun, Qing. "CHARACTERIZATION OF PHASE BEHAVIOR IN IDP SOLUTIONS BY UV-VIS SPECTROPHOTOMETRY AND PHASE VOLUME FRACTION MEASUREMENTS IN CAPILLARIES." (2021). https://digitalrepository.unm.edu/bme_etds/33