Biomedical Engineering ETDs

Publication Date

Summer 7-2018


This dissertation will present a progression from the detection of double-stranded DNA using a combination of toehold-mediated strand displacement and DNAzyme reactions in dilute saline solutions, to the generation of separate compartments to allow standardization of DNA computing elements, by protecting from complementary strands. In well-mixed solutions complementary regions cause spurious interactions. Importantly, these compartments also provide protection from nucleases. Along the way we will also explore the use of silica microsphere supported lipid bilayers to run compartmentalized DNA reactions on a fluid surface and the design of a molecule capable of DNA-based transmembrane signal transduction.




Molecular Computation, DNA Nanotechnologies, Diagnostics, Lipid Bilayers, Biomimicry

Document Type


Degree Name

Biomedical Engineering

Level of Degree


Department Name

Biomedical Engineering

First Committee Member (Chair)

Steven W. Graves

Second Committee Member

Darko Stefanovic

Third Committee Member

Matthew R. Lakin

Fourth Committee Member

Nick J. Carroll

Fifth Committee Member

Menake Piyasena