Biomedical Engineering ETDs
Publication Date
Summer 7-2018
Abstract
This dissertation will present a progression from the detection of double-stranded DNA using a combination of toehold-mediated strand displacement and DNAzyme reactions in dilute saline solutions, to the generation of separate compartments to allow standardization of DNA computing elements, by protecting from complementary strands. In well-mixed solutions complementary regions cause spurious interactions. Importantly, these compartments also provide protection from nucleases. Along the way we will also explore the use of silica microsphere supported lipid bilayers to run compartmentalized DNA reactions on a fluid surface and the design of a molecule capable of DNA-based transmembrane signal transduction.
Language
English
Keywords
Molecular Computation, DNA Nanotechnologies, Diagnostics, Lipid Bilayers, Biomimicry
Document Type
Dissertation
Degree Name
Biomedical Engineering
Level of Degree
Doctoral
Department Name
Biomedical Engineering
First Committee Member (Chair)
Steven W. Graves
Second Committee Member
Darko Stefanovic
Third Committee Member
Matthew R. Lakin
Fourth Committee Member
Nick J. Carroll
Fifth Committee Member
Menake Piyasena
Recommended Citation
Fabry-Wood, Aurora. "Compartmentalization of DNA-Based Molecular Computing Elements Using Lipid Bilayers." (2018). https://digitalrepository.unm.edu/bme_etds/20
