Biomphalaria Genome Initiative

• "Whole genome analysis of a schistosomiasis-transmitting freshwater snail," Nature Communications, 8, Article number: 15451 (2017), doi:10.1038/ncomms15451.

• "Proteomic analysis of Biomphalaria glabrata plasma proteins with binding affinity to those expressed by early developing larval Schistosoma mansoni, PLOS Pathogens, doi.org/10.1371/journal.ppat.1006081.
• "The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni," PLOS Neglected Tropical Diseases , doi.org/10.1371/journal.pntd.0005246.

Timeline

• 2015 Oct -- Publication process
• 2015 Oct Community-contributed analyses completed, manuscript submission
• 2013 WebApollo implemented by Vectorbase for Community annotation
• 2013 Vectorbase hosts, curates annotations and assembly
• 2013- MGI-supported conference calls support annotation effort
• 2012 Aug Formation steering committee, organization of internatinal annotation effort for Biomphalaria glabrata genome.
• 2012 Jun Biomphalaria glabrata genome assembly v4.3 (MGI)
• 2011 Jul MGI initiates Illumina sequencing of PE gDNA
• 2011 Jul MGI completes Illumina cDNA sequencing for 12 tissue types of Biomphalaria glabrata
• 2011 Jun MGI completes ~51x Illumina coverage short gDNA fragments.
• 2011 May MGI to obtain additional Illumina coverage to improve assembly
• 2011 Apr PRELIMINARY Biomphalaria glabrata genome assembly v4.0.1 (MGI)
• 2011 Mar MGI Illumina sequencing of 5 Gb gDNA
• 2010 Dec MGI to sequence Illumina paired ends (PE; 3kb; 8kb) for improved assembly
• 2010 Dec Illumina cDNA sequencing for 12 tissue types of Biomphalaria glabrata in progress.
• 2010 Jun PRELIMINARY Biomphalaria glabrata genome assembly v2.1 (MGI)
• 2009 Sep MGI LS454 paired end gDNA data (6Gbases), > 23x coverage in NCBI SRA
• 2009 Jul MGI LS454 gDNA data (17.1Gbases) in NCBI SRA
• 2009 Feb MGI releases full-length sequence data from several BACs to NCBI
• 2008 Jan MGI releases 100,304 BAC clone end sequences (BES) from the BG-BBa library to NCBI
• 2007 November: Biomphalaria glabrata genome project progress meeting at ASTMH 2007
• 2007 January: RNA, DNA template from selfed BB02 provided to initiate sequencing at MGI
• 2006 January: Over 46,000 nucleotide database entries for Biomphalaria glabrata
• 2005 October: Planning meeting for sequencing strategy at McDonnell Genome Institute MGI, St. Louis MO
• 2005 Planning meeting at NIH, Bethesda MD
• 2004 August: Biomphalaria glabrata prioritized as genomic sequencing target by NHGRI
• White paper proposal for genome sequencing Biomphalaria glabrata
• 2004 August: High quality BAC library (BG-BBa)
• 2004 January: Mitochondrial genome sequence of Biomphalaria glabrata completed
• 2003 September: Genome size for Biomphalaria glabrata published (check link below)
• 2002 October: Prioritization for BAC library publicly posted by NHGRI
• White paper proposal for BAC library Biomphalaria glabrata