Biology ETDs

Publication Date

5-1-1978

Abstract

The DNA synthesized during stable DNA replication (i.e., DNA replication in the absence of protein synthesis following a period of thymine starvation) was found to be alkali or RNase sensitive. Experiments were performed using both alkaline and neutral cell lysis and gradient sedimentation. Initial results under alkaline conditions revealed much smaller size DNA material than would normally be expected. This data was interpreted to show a reduced rate of DNA chain growth. However, under neutral conditions the DNA material was found to be nearly normal in size. The presence of an alkali-sensitive componant (RNA) was suspected and tested for with RNase under neutral conditions. Positive results were obtained. When the gradient sedimentation of DNA treated with alkali versus DNA under neutral conditions treated with RNase was compared, a size difference was still apparent. Therefore, it was believed an additional substance (dUMP) may be linked into the nascent DNA chain which could result in the creation of an alkali-sensitive site. This would occur upon removal of the uracil base by uracil-N­glycosylase.

Language

English

Document Type

Thesis

Degree Name

Biology

Level of Degree

Masters

Department Name

UNM Biology Department

First Committee Member (Chair)

Tokio Kogoma

Second Committee Member

Douglas E. Caldwell

Third Committee Member

Larry Lumir Barton

Download

Included in

Biology Commons

Share

COinS