Biology ETDs
Publication Date
7-10-1974
Abstract
The isolation of hypoxanthine-guanine phosphoribosyl transferase deficient mutants of Swiss mouse fibroblast cells was accomplished by exposure of large numbers of normal cells to the toxic antimetabolic agent 8-Azaguanine. Cell lines resistant to 0.1, 0.5 and 1.0 μg/ml of 8-Azaguanine were obtained. Enzyme studies of these cell lines revealed an inverse correlation between the level of resistance to 8-Azaguanine and the activity of the hypoxanthine-guanine phosphoribosyl transferase enzyme. One exceptional mutant has been isolated with a resistance to 8-Azaguanine and an enzyme activity two-fold higher than the parent cell. These mutants are discussed in terms of genetic and biochemical defects and indications for further studies are presented.
Language
English
Document Type
Thesis
Degree Name
Microbiology
Level of Degree
Masters
Department Name
UNM Biology Department
First Committee Member (Chair)
Thomas Irving Baker
Second Committee Member
David Lee Vander Jagt
Third Committee Member
Joseph Victor Scaletti
Recommended Citation
Sanford, Winston M.. "Isolation of Hypoxanthine-Guanine Phosphoribosyl Transferase Deficient Mutants in a Mammalian Cell Line." (1974). https://digitalrepository.unm.edu/biol_etds/544
