Transient de-coupling of photosynthesis and stomatal conductance in response to leaf primary vein cut in Helianthus annuus
Control of stomatal aperture is the primary way plants regulate gas exchange in the short-term, but what triggers stomatal responses to water stress is still debated. Chlorophyll-a fluorescence imaging, local leaf temperature, and gas exchange were measured simultaneously following a cut to primary leaf vein of Helianthus annuus to access the effect of local leaf xylem cavitation on leaf function. The treatment was repeated under 3 different vapor pressure deficit (VPD) conditions. Surprisingly, photosynthesis (A) and stomatal conductance (gs) responded inversely immediately following the treatment, indicating that A was not CO2 limited by stomatal closure. Comparisons of fluorescence images and temperature data showed that while both A and gs responded heterogeneously across the measured leaf area, local responses did not correspond spatially or temporally, suggesting that each was the result of a different mechanism and/or was initiated by a separate signal. Since the stomatal response varied with VPD but A did not, it is likely that only gs was ultimately responding to a hydraulic signal. Both A and gs recovered to near steady state levels by 900s after the cut. These results indicate that stomata respond immediately to a sudden hydraulic perturbation and that hydraulic redundancy in sunflower is sufficient to allow quick recovery to local interruption of vascular system. This experiment also provides evidence of transient de-coupling of A and gs following wounding.
stomata, electrical potential, heterogeneous behavior, photosynthesis, hydraulic conductance, hydraulic architecture
Level of Degree
UNM Biology Department
First Committee Member (Chair)
Second Committee Member
Green, Laura Elizabeth. "Transient de-coupling of photosynthesis and stomatal conductance in response to leaf primary vein cut in Helianthus annuus." (2009). https://digitalrepository.unm.edu/biol_etds/43