T cells are required to clear infection, and T cell motion plays a role in how quickly a T cell finds its target, from initial naive T cell activation by a dendritic cell to interaction with target cells in infected tissue. To better understand how different tissue environments affect T cell motility, we compared multiple features of T cell motion including speed, persistence, turning angle, directionality, and confinement of T cells moving in multiple murine tissues using microscopy. We quantitatively analyzed naive T cell motility within the lymph node and compared motility parameters with activated CD8 T cells moving within the villi of small intestine and lung under different activation conditions. Our motility analysis found that while the speeds and the overall displacement of T cells vary within all tissues analyzed, T cells in all tissues tended to persist at the same speed. Interestingly, we found that T cells in the lung show a marked population of T cells turning at close to 180
Torres DJ, Mrass P, Byrum J, Gonzales A, Martinez DN, Juarez E, Thompson E, Vezys V, Moses ME, Cannon JL. Quantitative analyses of T cell motion in tissue reveals factors driving T cell search in tissues. Elife. 2023 Oct 23;12:e84916. doi: 10.7554/eLife.84916. PMID: 37870221; PMCID: PMC10672806.