Health, Exercise, and Sports Sciences ETDs

Publication Date

Summer 7-14-2021


Purpose: To examine the effect of high intensity interval work (HIIW) and moderate intensity continuous work (MICW) patterns on markers of acute kidney injury (AKI) and kidney damage in a hot environment. Methods: Nine males completed two hours of physical work in a hot environment, performing either HIIW [2 minutes at 80% maximum oxygen consumption (VO2max) and 3 minutes at 30% VO2max] or MICW. Blood and urine samples were collected immediately before (Pre) and after (Post), one hour (1hr Post), and 24 hours after (24hr Post) the trials. Urine flow rate (UFR), osmolality (Uosm), and creatinine (uCreatinine), serum creatinine (sCreatinine), urinary neutrophil gelatinase-associated lipocalin (uNGAL) and urinary kidney injury marker 1 (uKIM-1) were measured. Estimated glomerular filtration rate (eGFR) was also calculated. Incidence rate of AKI were assessed using sCreatinine and the Acute Kidney Injury Network criteria. Results: 66.7% and 22% of participants developed Stage 1 AKI Post-Work immediately after HIIW and MICW, respectively. Serum creatinine was significantly higher during HIIW compared to MICW [F(1,8)= 63.720; p< 0.01] and eGFR was significantly lower during HIIW compared to MICW [F(1,8) 37.390; p< 0.01]. uKIM-1 and uNGAL displayed significant effects for time [F(3,24)= 15.508; pConclusion: When compared to MICW, HIIW cause a higher incidence rate of Stage 1 AKI in a hot environment, however there was no difference between the markers of kidney damage. It seems that higher intensity interval work impacts acute renal function, but not molecular markers of AKI (KIM-1 and NGAL).


acute kidney injury, heat stress, high intensity work, interval exercise, renal physiology

Document Type




Degree Name

Physical Education, Sports and Exercise Science

Level of Degree


Department Name

Health, Exercise, and Sports Sciences

First Committee Member (Chair)

Fabiano Amorim

Second Committee Member

Christine Mermier

Third Committee Member

Michael Deyhle

Fourth Committee Member

Orlando Laitano