Health, Exercise, and Sports Sciences ETDs

Publication Date

Spring 4-15-2021


It was previously hypothesized that endurance exercise training blunts the hypoxia inducible factor 1 (HIF-1) response to acute exercise. We investigated whether training status influences the skeletal muscle HIF-1 response to acute exercise. 7 endurance athletes and 8 untrained controls performed a bout of cycling exercise. Skeletal muscle protein levels of HIF-1α and its inhibitors, prolyl hydroxylase 2 (PHD2), factor inhibiting HIF (FIH) and von Hippel-Lindau (VHL) were measured pre, post and 3h post-exercise. HIF-1 target gene expression was assessed via rtPCR and RNA-sequencing. Baseline protein levels of FIH and PHD2 were greater in the trained group. The exercise-induced increase in HIF-1α was not different between groups. Pyruvate dehydrogenase kinase mRNA was higher in the trained group at baseline but was unaffected by exercise. RNA sequencing revealed no group differences in HIF-1 target genes. These data suggest that the HIF-1 response to exercise is not different between trained and untrained individuals.


HIF-1, gene, protein, RNA-seq


National Strength and Conditioning Association Foundation

Document Type




Degree Name

Physical Education, Sports and Exercise Science

Level of Degree


Department Name

Health, Exercise, and Sports Sciences

First Committee Member (Chair)

Christine Mermier

Second Committee Member

Fabiano Amorim

Third Committee Member

Michael Deyhle

Fourth Committee Member

Flávio de Castro Magalhães