Biomedical Engineering ETDs

Publication Date

Summer 7-14-2022


Open-source laboratory equipment has become an increasingly popular alternative to commercially available options due to its simple implementation, capability of user-customized modifications, and cost-effectiveness. Presented here is the development of a programmable and open-source fluidic system comprised of three custom-built syringe pumps for cell culture applications. The fluidic system was directly integrated with a multiwell plate on an environmentally controlled microscope platform for live-cell imaging and time-lapse analyses of cellular responses as culture conditions are adjusted using the programmable fluidic system. Proof-of-concept of this system is obtained by controlling autophagic activity in A549 cells by adjustment of nutrient supply. The programmability of this fluidic system allows for straightforward, low-cost implementation of feedback control methods in cell culture systems. These results will enable future research on understanding cell responses to time-dependent modulation of culture conditions or dosing with multiple pharmaceutical reagents.

Document Type


Degree Name

Biomedical Engineering

Level of Degree


Department Name

Biomedical Engineering

First Committee Member (Chair)

Dr. Andrew P. Shreve

Second Committee Member

Dr. Eva Chi

Third Committee Member

Dr. Nick Carroll