Shiga toxin-producing Escherichia coli (STEC) have been identified by the USDA as a serious threat to the nations health stemming from contaminations in the food supply, specifically, the beef chain. I have developed an assay that is able to screen samples for STEC in a rapid and multiplexed format. Multiplex oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples from the beef supply chain (farm to table) this assay will provide a multiplex and high throughput complement to the multiplex PCR assays currently in use. This research is focused on DNA detection of 8 STEC serotypes (STEC-8): O26, O45, O103, O104, O111, O121, O145, and O157:H7 as well as the virulence genes: stx1, stx2, and eae. The goal is to produce a multiplex panel of MOL-PCR probes for identifying DNA signatures corresponding to each of the STEC-8 serotypes and ultimately strain specific identification.'
MOL-PCR, Shiga toxin-producing E. coli, STEC
Level of Degree
This project was supported by Agriculture and Food Research Initiative Grant No. 2012-68003-30155 from the USDA National Institute of Food and Agriculture, Prevention, Detection and Control of Shiga Toxin-Producing Escherichia coli (STEC) from Pre-Harvest Through Consumption of Beef Products Program-A4101.
Woods, Travis. "Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli." (2015). https://digitalrepository.unm.edu/bme_etds/1