Neisseria gonorrhoeae (Ng) is a sexually transmitted bacterium that infects human reproductive mucosal surfaces. Due to the risk of complications in untreated cases and evolving antibiotic resistance, a prophylactic vaccine is critical for preventing infection. There are four Ng outer-membrane proteins that contribute to Ng survival and resistance: PorB, BamA, TbpA, and MtrE. Each protein contains surface-exposed loops that are targets of bactericidal antibodies. Vaccines were developed by displaying Ng epitopes on the surface of bacteriophage virus-like particles (VLP). VLPs are immunogenic, multivalent, self-assembling complexes composed of viral structural proteins. Antigenic peptide sequences can be displayed on the surface of VLPs via chemical conjugation or genetic insertion (recombination) into the structural protein sequence.
Thirty-seven VLP vaccines displaying Ng epitopes were constructed. Mice were vaccinated with VLPs, and antibody responses were measured. Immunogenicity studies demonstrated that the majority of VLPs elicited high-titer, peptide-specific antibody responses that may neutralize whole pathogen.
Vaccine, virus-like particle, Gonorrhea
Level of Degree
Biomedical Sciences Graduate Program
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Vaidya, Ashvini. "Developing a Virus-Like Particle (VLP)-Based Vaccine for Neisseria gonorrhoeae." (2020). https://digitalrepository.unm.edu/biom_etds/212
Bacterial Infections and Mycoses Commons, Community Health and Preventive Medicine Commons, Female Urogenital Diseases and Pregnancy Complications Commons, Male Urogenital Diseases Commons, Women's Health Commons