Biology ETDs

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Therapeutic lung lavage is a procedure by which the interior of the lung of a living animal is “washed” with a suitable solution. Broncho­pulmonary lavage, a special type of lung lavage in which the bronchioles and alveoli are washed, is the major concern of this thesis. Broncho­pulmonary lavage was investigated in 1920 as a possible treatment for lungs damaged by irritant gases and in the late 1950‘s as a method of hemodialysis. Currently, it is effectively used for the treatment of patients with obstructive lung disease and for the removal of radioactive materials from the mammalian lung. Bronchopulmonary lavage interferes with normal cardiopulmonary function. Among the factors which alter function are the instillation of saline into a lung and the unilateral hypoxia imposed as a result of filling one lung with saline. These factors were investigated in four Beagle dogs by comparing four different experimental conditions. These conditions were anesthesia alone (baseline), two methods of saline instillation, and unilateral hypoxia (with gas instead of saline in the hypoxic lung). A single lumen tracheal catheter was used in one method of saline instillation. In this method, the dog was hyperventilated prior to the relatively rapid introduction of saline into the lung. Few blood gas or shunt changes were observed in this technique, but heart rate, cardiac output, and systemic arterial pressure decreased from baseline anesthesia values. In the second method of saline instillation, the right and left lung airflows were separated by use of a double lumen tracheal catheter and the dog was not hype ventilated prior to the relatively slow instillation of saline into the right lung. In this technique, blood oxygen values decreased and venous admixture increased from baseline anesthesia; however, heart rate, cardiac output, and systemic arterial pressure remained constant. In the condition of unilateral hypoxia, a double lumen catheter was again used to separate right and left lung airflow. While the left lung breathed an anesthetic-oxygen mixture, the right lung was connected to a rebreathing bag and became hypoxic, Unilateral hypoxia resulted in physiologic changes that were similar to those of the double lumen method of saline instillation, suggesting that unilateral hypoxia was the major change producing factor during the double lumen lavage. In contrast, changes observed during the single lumen method were dissimilar to those of unilateral hypoxia. It appeared that most of the lung's perfused alveoli were gas filled during the single lumen lavage.



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UNM Biology Department

First Committee Member (Chair)

Marvin LeRoy Riedesel

Second Committee Member

Roger Orville McClellan

Third Committee Member

Paul Richard Kerkof

Fourth Committee Member

Bruce A. Muggenburg

Fifth Committee Member

Joe L. Mauderly

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