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The cytogenetic response of Beagle dog peripheral blood lymphocytes to inhaled insoluble yttrium-90 in fused clay was characterized and compared to that produced by whole-blood and whole-body irradiation with cobalt-60. Compartmental models were developed with digital and analog computers to define canine lymphocyte kinetics. From these data, the dose to the peripheral blood lymphocytes by 90y fused clay was estimated. Blood lymphocytes were cultured with phytohemagglutinin and incubated for 72 hr. Metaphase chromosomes were collected with colchicine. No significant chromosomal damage was observed to result from initial lung burdens less than 4. 8 mCi of 90y fused clay. Whole blood was exposed to y- radiation between 45 and 1230 rads. Whole-body bilateral exposures to y-radiation between 100 and 550 rads (average absorbed dose) were also performed and blood samples were obtained at 10 min and 9 days postexposure. The frequency of ring, dicentric, and isochromatid deletion chromsome aberrations increased at approximately the second power of the dose for in-vitro and in-vivo exposures to y- radiation when blood lymphocytes were cultured within a few minutes postirradiation. The aberration frequency at 9 days postexposure was very small at all levels of exposure and increased at approximately the first power of the dose.

Project Sponsors

The author was supported by a fellowship from the Associated Western Universities Inc. The research was performed at the Love­lace Foundation in Albuquerque, New Mexico, under Contract No. AT(29-2)-1013 between the United States Atomic Energy Commission and the Lovelace Foundation for Medical Education and Research.



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Degree Name


Level of Degree


Department Name

UNM Biology Department

First Committee Member (Chair)

Marvin LeRoy Riedesel

Second Committee Member

Clarence Clayton Hoff

Third Committee Member

Gordon Verle Johnson

Fourth Committee Member

Antone L. Brooks

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