Biology ETDs

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The incubation of chromium (51Cr)-labeled red blood cells (RBC) at 37° C in 5% CO2 in the presence of hemozoin, a malarial pigment, resulted in the release of 51Cr depending upon the species of Plasmodium (rodent or simian) from which the hemozoin was prepared. Hemozoin prepared from Plasmodium berghei caused specific release of 26% of the label from RBC of Lewis rats of age 4 months or older. No significant effect was seen in younger rats since the control RBC's released 55-65% of the label spontaneously. This same hemozoin preparation caused inhibition of release of the label when incubated with RBC of immune CF1 mice. Again hemozoin had no effect on RBC of normal mice, as there was a spontaneous release (70-80%) of the label during incubation. Hemozoin prepared from P. knowlesi caused a specific release of 35% of the label when incubated with labeled Lewis rat RBC's either infected or normal. No increase release of 51Cr was observed when hemozoin was incubated with RBC's from normal Rhesus monkeys. In all cases where release or inhibition of the label occurred, the reaction was dose dependent.



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Department Name

UNM Biology Department

First Committee Member (Chair)

Paul R. Kerkof

Second Committee Member

Paul H. Silverman

Third Committee Member

Sei Tokuda

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Biology Commons