Biomedical Engineering ETDs
Publication Date
Summer 7-14-2022
Abstract
Open-source laboratory equipment has become an increasingly popular alternative to commercially available options due to its simple implementation, capability of user-customized modifications, and cost-effectiveness. Presented here is the development of a programmable and open-source fluidic system comprised of three custom-built syringe pumps for cell culture applications. The fluidic system was directly integrated with a multiwell plate on an environmentally controlled microscope platform for live-cell imaging and time-lapse analyses of cellular responses as culture conditions are adjusted using the programmable fluidic system. Proof-of-concept of this system is obtained by controlling autophagic activity in A549 cells by adjustment of nutrient supply. The programmability of this fluidic system allows for straightforward, low-cost implementation of feedback control methods in cell culture systems. These results will enable future research on understanding cell responses to time-dependent modulation of culture conditions or dosing with multiple pharmaceutical reagents.
Document Type
Thesis
Degree Name
Biomedical Engineering
Level of Degree
Masters
Department Name
Biomedical Engineering
First Committee Member (Chair)
Dr. Andrew P. Shreve
Second Committee Member
Dr. Eva Chi
Third Committee Member
Dr. Nick Carroll
Recommended Citation
Montoya, Charles. "Development of a Programmable, Open-Source, and Cost-Effective Fluidic System for Cell Culture Applications." (2022). https://digitalrepository.unm.edu/bme_etds/35
Included in
Biomedical Devices and Instrumentation Commons, Other Medicine and Health Sciences Commons
