Biomedical Engineering ETDs

Publication Date

Fall 12-15-2017

Abstract

Astounding success of Human genome project and accelerating success of sequencing technologies have enabled $ 1000 genome goals possible. But, this is still far-fetched from the reach of many resource refrained populations with high genetic variations causing lethal genetic diseases. Based on present technology principles, I have developed prototypes for affordable, scalable and customizable point-of-care genotyping and targeted sequencing. Ion-sensitive field effect transistors with novel read-out and signal amplification techniques are used for laying foundation of possible ISFET based allele-arrays. Sequencing-by-synthesis based full-fledge sequencer is made with novel immobilization, flow-cell and data acquisition methods for massive sequenciing.

Language

English

Keywords

Genotyping, affordable, dna sequencing, targeted sequencing

Document Type

Dissertation

Degree Name

Biomedical Engineering

Level of Degree

Doctoral

Department Name

Biomedical Engineering

First Committee Member (Chair)

Dr. Jeremy Edwards

Second Committee Member

Dr. Elizabeth Dirk

Third Committee Member

Dr. Heather Canavan

Fourth Committee Member

Dr. Payman Zarkesh-Ha

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