The processes of vesicular trafficking and membrane fusion are fundamental to nervous system development and communication among neurons within integrated circuits. The regulated release of several neurotransmitters is dependent on synaptosomal-associated protein 25 kDa (SNAP-25)-containing soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) core complexes. The requirement for this fusogenic machinery in evoked neurosecretion has been repeatedly demonstrated through the use of mutant mouse models and neurotoxin blockades. However, the existence of a functional role for a SNAP-25-containing SNARE complex has not been shown in the major inhibitory neurotransmitter system of the brain, gamma-aminobutyric acid (GABA). To determine whether SNAP-25 participates in the evoked GABAergic neurotransmission, we investigated the expression and function of this protein in inhibitory terminals. In addition, we identified the major SNAP-25 isoform expressed by mature GABAergic neurons. The results presented here provide compelling evidence that SNAP-25 is critical for evoked GABA release and is expressed in the presynaptic terminals of mature GABAergic neurons, consistent with its function as a component of a fundamental core SNARE complex required for stimulus-driven neurotransmission. Furthermore, we conclude that SNAP-25b is the predominant isoform expressed in central inhibitory neurons of the adult brain.
SNAP-25 presynaptic terminal GABA SNARE glutamatergic synaptic vesicle recycling neurotoxin exocytosis
Level of Degree
Biomedical Sciences Graduate Program
Wilson, Michael C.
First Committee Member (Chair)
Second Committee Member
Cunningham, Lee Anna
Third Committee Member
Tafoya, Lawrence C. R.. "SNAP-25 is a component of a ubiquitous SNARE complex required for evoked neuroexocytosis in GABAergic neurons." (2011). http://digitalrepository.unm.edu/biom_etds/33