Energize New Mexico
National Science Foundation
Metabolic response to four suboptimal cultivation temperatures was explored in Nannochloropsis salina, with an emphasis on fatty acid and lipid metabolism. Cultures were cultivated in controlled environmental photobioreactors. Physiological performance, characterized by growth rate and photosynthetic output, were reduced at all three cold stress temperatures (5 degrees Celsius, 10 degrees Celsius, 15 degrees Celsius). However, biomass productivity and overall growth for cultures at 20 degrees Celsius was like that of those grown at the optimal temperature (25 degrees Celsius). Fatty acid productivity was elevated at all suboptimal temperatures, and both total fatty acid and eicosapentaenoic acid content were statically elevated at 20 degrees Celsius. Lipid accumulation was observed exclusively in response to cold stress; however, lipid turnover and enrichment in polyunsaturated fatty acids occurred in all temperature variants. In addition, extensive lipid remodeling occurred in both polar and neutral lipid pools, specifically in monogalactosyl diacylglycerol and triacylglycerol pools. While 20 degrees Celsius is optimal for eicosapentaenoic acid and total fatty acid productivity, cold stress is necessary to induce both productivity and polyunsaturated fatty acid enrichment in triacylglycerol pools.
Holguin, Omar. "Retention Index Data." (2018). https://digitalrepository.unm.edu/energizenm/686