Biology ETDs

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The DNA synthesized during stable DNA replication (i.e., DNA replication in the absence of protein synthesis following a period of thymine starvation) was found to be alkali or RNase sensitive. Experiments were performed using both alkaline and neutral cell lysis and gradient sedimentation. Initial results under alkaline conditions revealed much smaller size DNA material than would normally be expected. This data was interpreted to show a reduced rate of DNA chain growth. However, under neutral conditions the DNA material was found to be nearly normal in size. The presence of an alkali-sensitive componant (RNA) was suspected and tested for with RNase under neutral conditions. Positive results were obtained. When the gradient sedimentation of DNA treated with alkali versus DNA under neutral conditions treated with RNase was compared, a size difference was still apparent. Therefore, it was believed an additional substance (dUMP) may be linked into the nascent DNA chain which could result in the creation of an alkali-sensitive site. This would occur upon removal of the uracil base by uracil-N­glycosylase.



Document Type


Degree Name


Level of Degree


Department Name

UNM Biology Department

First Committee Member (Chair)

Tokio Kogoma

Second Committee Member

Douglas E. Caldwell

Third Committee Member

Larry Lumir Barton

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Biology Commons