The Effect of Coxiella Burnetii Parasitism on the Fertile Chieken Egg Injected with Glucose-14C

Author

Oswald G. Baca

Publication Date

5-16-1969

Abstract

The effect of parasitism by the rickettsia Coxiella burnetii on the chicken embryo was studied by extracting rickettsiae separated from infected yolk sacs and examining the extracts for radioactivity arising from D-glucose-U-¹⁴C injected into the embryonated eggs. The rickettsiae were separated from the yolk sacs with a cation exchange resin and differential centrifugation. The purified rickettsiae were then extracted for various components. The extracts 4 were subjected to radioactivity determination with an internal sample liquid scintillation counter. The extracts were also chromatographed and superimposed on X-ray film for radioautography. No significant differences in radioactivity were found be tween the experimental s and control extracts. There was a lower amount of radioactivity in unpurified infected yolk sacs than in those that were uninfected.

Language

English

Document Type

Thesis

Degree Name

Biology

Level of Degree

Masters

Department Name

UNM Biology Department

First Committee Member (Chair)

John Beakley

Second Committee Member

James Booth

Third Committee Member

William C. Martin

Recommended Citation

Baca, Oswald G.. "The Effect of Coxiella Burnetii Parasitism on the Fertile Chieken Egg Injected with Glucose-14C." (1969). https://digitalrepository.unm.edu/biol_etds/305