Cellular differentiation is an important process for the development and maintenance of an organism. Saccharomyces cerevisiae (yeast) has been successful as a tool for important discoveries relating to eukaryotic cellular processes. Stationary phase yeast cultures have been found to differentiate into two major cell types with distinct phenotypes. This genome-wide study using pooled samples of the yeast homozygous deletion set has identified over 400 genes which are important for differentiation of stationary phase yeast. These genes are predominantly involved in mitochondrial function thereby supporting our hypothesis that mitochondrial function is integral to the differentiation process. In addition, further experimentation focused on viability of yeast cultures revealed the importance of vacuolar proteins in survival of yeast cultures.
"yeast, saccharomyces, differentiation, culture, genomics"
Louis Stokes Alliance for Minority Participation; Initiatives to Maximize Student Diversity
Level of Degree
Biomedical Sciences Graduate Program
First Committee Member (Chair)
Second Committee Member
Manzanilla, Elaine. "Identification of genes required for differentiation of quiescent and nonquiescent cells in stationary phase cultures of Saccharomyces cerevisiae." (2013). http://digitalrepository.unm.edu/biom_etds/72