Biomedical Sciences ETDs

Publication Date

7-1-2013

Abstract

Cellular differentiation is an important process for the development and maintenance of an organism. Saccharomyces cerevisiae (yeast) has been successful as a tool for important discoveries relating to eukaryotic cellular processes. Stationary phase yeast cultures have been found to differentiate into two major cell types with distinct phenotypes. This genome-wide study using pooled samples of the yeast homozygous deletion set has identified over 400 genes which are important for differentiation of stationary phase yeast. These genes are predominantly involved in mitochondrial function thereby supporting our hypothesis that mitochondrial function is integral to the differentiation process. In addition, further experimentation focused on viability of yeast cultures revealed the importance of vacuolar proteins in survival of yeast cultures.

Keywords

"yeast, saccharomyces, differentiation, culture, genomics"

Sponsors

Louis Stokes Alliance for Minority Participation; Initiatives to Maximize Student Diversity

Document Type

Thesis

Language

English

Degree Name

Biomedical Sciences

Level of Degree

Masters

Department Name

Biomedical Sciences Graduate Program

First Advisor

Werner-Washburne, Margaret

First Committee Member (Chair)

Thompson, Todd

Second Committee Member

Natvig, Donald

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