A method for the colorimetric estimation of adrenaline has been developed which makes it possible to determine the adrenaline in the presence of other dihydroxypheylamine compounds, specifically DOPA and tyrosine.
The method employed is a modification of the procedure of Sivadjian, using sodium chloride and hydrogen peroxide in the presence of related compounds, semicarbazide.
The method discussed is more specific than those in the literature but not as sensitive. The lower limit of the method is 25ᴽ, which is accurate to a plus or minus 5 per cent.
Data are presented to determine the optimum pH and standard concentrations for the method to be effective. Data are discussed with the view to determine the mechanisms involved in this color production.
Level of Degree
UNM Biology Department
First Committee Member (Chair)
Second Committee Member
Richard B. Johnson
Third Committee Member
Martin William Fleck
Steinback, Glenn H.. "A Method for the Determination of Adrenaline in the Presence of Mono and Dihydroxy Compounds." (1951). http://digitalrepository.unm.edu/biol_etds/246